| Scan head input/output port |
3 laser input ports |
| 3 signal output ports for standard, spectral and third-party detectors (FCS/FCCS/FLIM) |
| Laser for multiphoton microscopy |
Compatible laser |
Mai Tai HP/eHP DeepSee (Newport Corp.) |
| Chameleon Vision II (Coherent Inc.) |
| Modulation |
Method: AOM (Acousto-Optic Modulator) device |
| Control: power control, return mask, ROI exposure control |
| Incident optics |
700-1080 nm, auto alignment |
| Laser for confocal microscopy (option) |
Compatible laser |
405 nm, 440/445 nm, 488 nm, 561/594 nm, 638/640nm, Ar laser (457 nm, 488 nm, 514 nm), HeNe laser (543 nm) |
| Modulation |
Method: AOTF (Acousto-Optic Tunable Filter) or AOM (Acousto-Optic Modulator) device |
| Control: power control for each wavelength, return mask, ROI exposure control |
| Laser unit |
LU-N4/N4S Laser Unit 405/488/561/640 (AOTF modulation), LU-N3 Laser Unit405/488/561 (AOTF modulation), |
| LU4A 4-laser module A, C-LU3EX 3-laser module EX |
| NDD for multiphoton microscopy |
Wavelength |
380-650 nm |
| Detector |
4 PMTs |
| Filter cube |
492SP, 525/50, 575/25, 629/53, DM458, DM495, DM511, DM560, DM593 |
| — |
| Detector type |
Episcopic NDD (for Ni-E/FN1/Ti-E), Episcopic GaAsP NDD (for Ni-E/FN1/Ti-E), Diascopic NDD (for Ni-E only), Diascopic GaAsP NDD (for Ni-E, FN1) |
| — |
| Standard fluorescence detector (option) |
Wavelength |
400-750 nm (400-650 nm for multiphoton observation) |
| Detector |
A1-DU4 4 Detector Unit: 4 normal PMTs |
| A1-DUG GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 normal PMTs |
| Filter cube |
6 filter cubes commonly used for a microscope mountable on each of three filter wheels |
| Recommended wavelengths for multiphoton/confocal observation: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 595/50, 700/75 |
| Diascopic detector (option) |
Wavelength |
440-645 nm |
| Detector |
PMT |
| FOV |
Square inscribed in a ø18 mm circle |
| Image bit depth |
4096 gray intensity levels (12 bit) |
| Scan head |
Standard image acquisition |
Scanner: galvano scanner x2 |
| Pixel size: max. 4096 x 4096 pixels |
| Scanning speed: |
| Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi-direction) |
| Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction)*2 |
| Zoom: 1-1000x continuously variable |
| Scanning mode: X-Y, X-T, X-Z, XY rotation, Free line |
| High-speed image acquisition |
— |
| IR laser wavelength range |
700 to 1000 nm |
| Dichroic mirror |
Low-angle incidence method |
| Position: 8 |
| Standard filter: 405/488, 405/488/561, 405/488/561/638, 400-457/514/IR, 405/488/543/638, BS20/80, IR, 405/488/561/IR |
| Pinhole |
12-256 μm variable (1st image plane) |
| Spectral detector*3 (option) |
Wavelength detection range |
400-750nm (400-650nm for multiphoton observation) |
| Number of channels |
32 channels |
| Spectral image acquisition speed |
4 fps (256 x 256 pixels), 1000 lps |
| Wavelength resolution |
80 nm (2.5 nm), 192 nm (6 nm), 320 nm (10 nm) |
| Wavelength range variable in 0.25 nm steps |
| Unmixing |
High-speed unmixing, Precision unmixing |
| Compatible microscopes |
ECLIPSE Ti-E inverted microscope, ECLIPSE FN1 fixed stage microscope, ECLIPSE Ni-E upright microscope (focusing nosepiece type and focusing stage type) |
| Z step |
Ti-E: 0.025 μm, FN1 stepping motor: 0.05 μm |
| Ni-E: 0.025 μm |
| Option |
Motorized XY stage (for Ti-E/Ni-E), High-speed Z stage (for Ti-E), High-speed piezo objective-positioning system (for FN1/Ni-E) |
| Software |
Display/image generation |
2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing |
| Image format |
JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS |
| Application |
FRAP, FLIP, FRET (option), photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization |
| Control computer |
OS |
Microsoft Windows®7 Professional 64 bits SP1 |
| CPU |
Intel Xeon E5-2667 (2.90 GHz/15 MB/1666 MHz) or higher |
| Memory |
16 GB (4 GB x 4 + additional 2 GB x3) |
| Hard disk |
300 GB SAS (15,000 rpm) x2, RAID 0 configuration |
| Data transfer |
Dedicated data transfer I/F |
| Network interface |
10/100/1000 Gigabit Ethernet |
| Monitor |
1600 x 1200 or higher resolution, dual monitor configuration recommended |
| Vibration isolated table |
1800 (W) x 1500 (D) mm required (for FN1/Ni-E), or 1500 (W) x 1500 (D) mm required (for Ti-E) |
