| Input/output Port |
3 laser input ports |
| 4 signal output ports (for 4-PMT detector, spectral detector, VAAS, optional detector*1) |
| Laser for Confocal Microscopy |
|
| Compatible Laser |
Laser diode 405 nm, 440/445 nm, Ar laser (457 nm/488nm/514 nm,), Solid-state laser 488 nm, 561/594 nm, G-HeNe laser 543 nm, Laser diode 638/640 nm |
| Modulation |
Method: AOTF (Acousto-Optical Tunable Filter) or AOM (Acousto-Optical Modulator) device |
| Control: power control for each wavelength, return mask, ROI exposure control |
| Laser Unit |
Standard: 4-laser module A or 3-laser module EX |
| Optional: 3-laser module EX (when 4-laser module is chosen as standard laser unit) |
| Laser for Multiphoton Microscopy |
|
| Compatible Laser |
Mai Tai HP/eHP DeepSee (Newport Corp.) |
| Chameleon Vision II (Coherent Inc.) |
| Modulation |
Method: AOM (Acousto-Optical Modulator) device |
| Control: power control, return mask, ROI exposure control |
| Incident Optics |
700-1000 nm, auto alignment |
| Standard 4-Channel Detector |
|
| Wavelength |
400-750 nm (400-650 nm for multiphoton observation) |
| Detector |
4 PMT |
| Filter Cube |
6 filter cubes commonly used for a microscope mountable on each of three filter wheels |
| Recommended wavelengths for multiphoton/confocal observation: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 595/50, 700/75 |
| Diascopic Detector |
|
| Wavelength |
440-645 nm |
| Detector |
PMT |
| NDD for Multiphoton Microscopy |
|
| Wavelength |
400-650 nm |
| Detector |
4 PMT |
| Filter Cube |
Filter cubes commonly used for a microscope |
| Recommended filter sets for multiphoton: 492SP, 525/50, 575/25, 692/53, DM458, DM495, DM511, DM560, DM593 |
| Detector Type |
Episcopic NDD (for Ni-E/FN1/Ti-E) |
| Diascopic NDD (for Ni-E) |
| Episcopic GaAsP NDD (for FN1) |
| Unmixing |
Channel unmixing |
| Image Bit Depth |
4096 gray intensity levels (12 bit) |
| Scanning Head |
|
| Scanning |
FOV: Square inscribed in a ∅18 mm circle |
| Standard image acquisition |
| Scanner: non-resonant scanner x2 |
| Pixel size: max. 4096 x 4096 pixels |
| Scanning speed |
| Standard Mode: 2 fps (512 x 512 pixels, bi-direction), max. 24 fps (512 x 32 pixels bi-direction) |
| Fast Mode: 10fps (512 x 512 pixels, bi-direction), max. 130fps (512 x 32 pixels, bi-direction)*2 |
| Zoom: 1-1000x continuously variable |
| Scanning mode: X-Y, X-T, X-Z, XY rotation, Free line |
| High-speed image acquisition |
| Scanner: resonant scanner (X-axis, resonance frequency 7.8 kHz), non-resonant scanner (Y-axis) |
| Pixel size: max. 512 x 512 pixels |
| Scanning speed: 30 fps (512 x 512 pixels) to 420 fps (512 x 32 pixels), |
| 15,600 lines/sec (line speed) |
| Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x) |
| Scanning mode: X-Y, X-T, X-Z |
| Acquisition method: Standard image acquisition, High-speed image acquisition, Simultaneous photo activation and image acquisition |
| Dichroic Mirror |
Low-angle incidence method |
| Position: 8 |
| Standard filter: 405/488, 405/488/561, 405/488/561/638, 400-457/514/IR, 405/488/543/638, BS20/80, IR, 405/488/561/IR |
| Pinhole |
12-256 μm variable (1st image plane) |
| Wavelength Detection Range |
400 nm-750 nm (400 nm-650 nm with multiphoton microscopy) |
| Number of Channels |
32 channels |
| Spectral Image Acquisition Speed |
4 fps (256 x 256 pixels), 1000 lps |
| Wavelength Resolution |
80nm (2.5 nm), 192nm (6 nm), 320nm (10 nm) |
| Wavelength range variable in 0.25 nm steps |
| Unmixing |
High-speed unmixing, Precision unmixing |
| Compatible Microscopes |
ECLIPSE Ti-E inverted microscope |
| ECLIPSE FN1 fixed stage microscope |
| ECLIPSE Ni-E upright microscope (focusing nosepiece type and focusing stage type) |
| Z Step |
Ti-E: 0.025 μm, FN1 stepping motor: 0.05 μm, Ni-E: 0.025 μm |
| Option |
Motorized XY stage (for Ti-E/Ni-E), High-speed Z stage (for Ti-E), High-speed piezo objective-positioning system (for FN1/Ni-E), VAAS |
| Software |
|
| Display/Image Generation |
2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing |
| Image Format |
JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS |
| Application |
FRAP, FLIP, FRET, photo activation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization |
| OS |
Microsoft Windows® 7 Professional 64-bit SP1 (Japanese version /English version) |
| CPU |
Intel Xeon X5672 (3.20 GHz/8 MB/1333 MHz Quad Core) |
| Memory |
12 GB |
| Hard Disk |
SAS (15,000 rpm), 300 GB x2, RAID 0 configuration |
| Data Transfer |
Dedicated data transfer I/F |
| Monitor |
1600 x 1200 or higher resolution, dual monitor configuration recommended |
| Vibration Isolated Table |
180 |
