Mô tả sản phẩm: VELOCITY DNA Polymerase Bioline
Features
- High-speed, high-fidelity DNA polymerase
- High processivity
- Fast amplification
- Shorter PCR runs for longer templates
- Robust, requires minimal optimization of the reaction
Applications
- Cloning techniques where high-fidelity is desirable
- Blunt-end cloning
- Amplification of difficult templates
- Mutagenesis
Description
VELOCITY is a fast thermostable enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities. VELOCITY provides high-fidelity with an error-rate of 4.4 x 10-7 combined with a high speed DNA synthesis as fast as 15s/kb for templates of up to 5kb and 30s/kb for templates longer than 5kb. The inherent high speed DNA synthesis is due to the enhanced processivity of the polymerase
VELOCITY is easy to use since it works with many different protocols and requires minimal optimization. The polymerase produces higher yields than most commercially available enzymes and generates blunt-ended amplicons. The error rate of VELOCITY DNA Polymerase (4.4 x 10-7) was determined using the rpsL fidelity assay (Lackovich et al., 2001; Fujii et al., 1999).
Components
- VELOCITY DNA Polymerase
- 5x Hi-Fi Buffer (contains 10mM Mg2+)
- 50mM MgCl2 Solution
- DMSO
Working Concentration
One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
Concentration
2u/µl
Storage Conditions
VELOCITY DNA Polymerase can be stored for 12 months at –20°C.
Shipping Conditions
On Dry Ice or Blue Ice.
Product Citations
- Killelea, T. & Connolly, B.A. Chem. BioChem. 12, 1330–1336 (2011).
- Thornton, J.K., et al. PLoS ONE 6(8), e23878 (2011).
- Paxton, C.W., et al. Am. J. Physiol. 300, C1345-C1355 (2011).
- Renaud, C., et al. J. Clin. Virol. 49(1), 21-25 (2010).
- Huang, X.X., et al. Am. J. Pathol. 174(4), 1534–1543 (2009).
- Norgate, M., et al. PLoS One. 4(11), e7950 (2009).
