Mô tả sản phẩm: Uracil DNA Glycosylase Bioline
Features
- Removal of uracil from uracil containing DNA
- Novel temperature sensitive mutant irreversibly inactive after heating
Applications
- UDG treatment of uracil-containing DNA to prevent amplificaton by DNA polymerases.
- Used to investigate features of protein – DNA interactions
Description
Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single– or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases). UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200mM).
Bioline UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.
Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.
Components
- Uracil DNA Glycosylase
- 10x Reaction Buffer
Unit Definition
One unit is the amount of enzyme that catalyzes the release of 60µmol of uracil per minute from uracil-containing double-stranded DNA.
Concentration
1u/µl
Storage Conditions
Uracil DNA Glycosylase can be stored for 6 months at –20°C.
Product Citations
- Rashtchian, A., et al. PCR methods and applications 2(2), 124-130 (1992).
- Stivers, J.T. & Drohat, A.C. Archives of Biochemistry and Biophysics 396(1), 1-9 (2001).
