MyTaq™ Extract-PCR Kit Bioline

Features and Benefits

• Rapid extraction protocol - High-yield PCR-ready DNA in 15 minutes
• Replaces complicated DNA extraction procedures
• Convenient single-tube extraction minimizes contamination
• Coupled with MyTaq™ HS Red Mix for fast and highly-specific amplification

Applications

• Ideal for high-throughput genotyping from mammalian tissues
• Detection of transgenes
• Knockout analysis

Description
Many DNA extraction methods can be laborious and time consuming, involving phenol extraction, overnight incubations often combined with column purification. MyTaq Extract-PCR Kit offers a rapid and easy alternative for the extraction and amplification of DNA from a variety of tissue types, particularly solid tissues such as mouse tail (fig. 1) or mouse ear. The DNA extractions are performed in a single-tube, without the need for multiple washing steps, greatly reducing the risk of sample loss and contamination. 

The extracted DNA is amplified using MyTaq HS Red Mix, the latest generation of very high performance polymerase mastermixes unique to Bioline, powered by antibody mediated hot-start to reduce non-specific amplification. The advanced formulation of MyTaq HS Red Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity or yield (fig. 2). 

Together, the single tube lysis protocol and MyTaq HS Red Mix maximizes sensitivity, while minimizing contamination risk, significantly reduces reaction times as well as delivering improved success rates in protocols such as mouse DNA characterization. The specially designed MyTaq HS Red formulation does not interfere with the PCR reaction but allows users to load samples directly onto a gel after the PCR without the need to add loading buffer. The red dye migrates like a 350bp fragment on a 2% agarose TAE gel (or 600bp on a 1% agarose).

Components

Buffer A

Buffer B

MyTaq HS Red Mix (2x)

Storage Conditions
MyTaq Extract-PCR Kit is shipped on ice packs. On arrival store at -20°C for optimum stability. Repeated freeze/thaw cycles should be avoided.