BIO-X-ACT™ Short DNA Polymerase Bioline

Features

• Ideal for problematic templates
• Ideal for templates that fail with standard Taq DNA Polymerases
• Higher fidelity than Taq due to proofreading activity
• Amplifies fragments up to 5Kb
• Available as a ready-to-use 2x reaction mix (BIO-X-ACT™ Short Mix)

Applications

• High-fidelity PCR
• Suitable for TA cloning
• GC-rich templates
• Crude sample PCR
• Forensic applications

Description
BIO-X-ACT™ Short DNA Polymerase is a high-performance enzyme designed for difficult or problematic PCR applications requiring high processivity and fidelity. Two versions of BIO-X-ACT are available, for amplification of short and long DNA fragments respectively, and are the polymerases of choice for difficult applications that would normally fail with standard Taq polymerases. 

BIO-X-ACT Short is recommended for short genomic DNA fragments of up to 2Kb, or Lambda DNA fragments up to 5Kb. Using Lambda DNA as template, the best performance is achieved within the 100bp-3Kb range. 

For enhanced specificity, BIO-X-ACT Short is supplied with a vial of 5x Hi-Spec Additive. Hi-Spec Additive is a very efficient enhancer, which helps to prevent the formation of false background bands and smearing. The specificity and performance can be further improved with the use of 2x PolyMate Additive (not supplied, see associated products), which is designed for GC or AT-rich DNA, "dirty" templates or sequences with difficult melting profiles.

Components

• BIO-X-ACT Short DNA Polymerase
• 10x NH4 Buffer
• 50mM MgCl₂ Solution
• 5x Hi-Spec Additive

Unit Definition
One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.

Concentration
4u/µl

Storage Conditions
BIO-X-ACT Short DNA Polymerase can be stored for 12 months at -20°C.

Shipping Conditions
On Dry Ice or Blue Ice.

Product Citations

1. Blake, D.P., et al. PLoS Pathog. 7(2), e1001279 (2011).
2. Kuperman, Y. et al. Mol. Endocrinol. 25 157-169 (2011).
3. Li, H., et al. J. Bio. Chem. 286, 21500-21510 (2011).
4. Taliaz, D., et al. J. Neurosci. 31 4475-4483 (2011).
5. Boura-Halfon, S., et al. Mol. Endocrinol. 24 2179-2192 (2010).
6. David, M., et al. RNA 16 364-374 (2010).
7. Falvella, F. S., et al. J. Natl. Can. Inst. 102(17), 1367-1370. (2010).
8. Petersen, H. J., et al. Infect. Immun. 78, 413-422 (2010).
9. Matic, M., et al. Intl. J. Biochem. Cell Biol. 42(5), 672-682 (2010).
10. Lim, T. S., et al. New Biotechnol. 27(2), 108-117 (2010).
11. Solomon, E., et al. J. Bio. Chem. 285, 21969-77 (2010).
12. Hedman, J., et al. BioTechniques 47, 951-958 (2009).
13. Dhivya, K. & Ghosh, G. J. Forensic Dental Sci. 1(2), 104-106 (2009).