Mô tả sản phẩm: MangoTaq™ DNA Polymerase Bioline
"The best performance was exhibited by the MangoTaq DNA polymerase, which was the only polymerase which was able to amplify the 620bp amplification product from the 102 year old sample."
- University of Hohenheim, Stuttgart, Germany
Read the PloS One paper here
Features
- Excellent price and performance
- Easy visual recognition
- Direct loading onto agarose gels
- Robust performance
- Available as a ready-to-use 2x Reaction mix (MangoMix™)
Applications
- High throughput applications
- Suited to a wide range of PCR assays
- Products suitable for TA cloning
Description
MangoTaq™ DNA Polymerase is a formulation of Taq DNA Polymerase which offers high yield across a wide range of DNA concentrations (Fig. 1). MangoTaq DNA Polymerase possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors. The polymerase is supplied with two different reaction buffers for greater user flexibility. For high-throughput applications, MangoTaq and the colored reaction buffer make an ideal choice, since this combination enables the user to load directly on a gel in order to facilitate easy recognition.
The two reaction buffers supplied are: 5x Colored Reaction Buffer and 5x Colorless Reaction Buffer. The colored reaction buffer contains red and orange dyes, which separate during electrophoresis and provide quick reference points for monitoring the mobility of the DNA samples in the gel. The colored reaction buffer can be loaded directly onto an agarose gel for analysis, without the need for separate gel-loading buffer. The presence of the dyes has no effect on routine enzymatic manipulations, although rare exceptions may exist.
Since the colorless reaction buffer does not contain reference dyes, it is suitable for use when reaction products will be used directly for down-stream processes involving absorbance or fluorescent detection. The specificity and performance of MangoTaq DNA Polymerase can be further improved with the use of 2x PolyMate Additive, which is designed for GC or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.
Components
- MangoTaq DNA Polymerase
- 5x MangoTaq Buffer, colored
- 5x MangoTaq Buffer, colorless
- 50mM MgCl2 Solution
Unit Definition
One unit will incorporate 10nmoles of dNTPs in 30min at 72°C
Concentration
5u/µl
Storage Conditions
MangoTaq can be stored for 12 months at –20°C.
Shipping Conditions
On Dry Ice or Blue Ice
Product Citations
- Tracy, L. N. & Jamieson, I. G. Conserv. Genet. 12(2), 517-526 (2011).
- Shi, A., et al. Am. J. Biotechnol. Mol. Sci. DOI:10.5251/ajbms.2011.1.1.8.16 (2011).
- Lau, A., et al. J. Clin. Microbiol. 48(3), 811–816 (2010).
- Thines, M., et al. Eur. J. Plant Path. 128(1), 81-89 (2010).
- Knower, K. C., et al. Mol. Cell. Endo. 321(2), 123-130 (2010).
- Nolan, M. J., et al. Infection, Gene. Evol. 10(8), 1179-1187 (2010).
- Yeung, N. W., et al. Biol. J. Linnean Soc. 98, 586–595 (2009).
- Telle, S. & Thines, M. PLoS One 3(10) e3584 (2008).
- Lee, R. M., et al. Weed Sci. 56(3), 371-375 (2008).
